Cysteine Promoted C-Terminal Hydrazinolysis of Native Peptides and Proteins
نویسندگان
چکیده
منابع مشابه
Cysteine Promoted C-Terminal Hydrazinolysis of Native Peptides and Proteins**
Tagging the terminus: N→S acyl transfer in native peptides and proteins can be reliably intercepted with hydrazine. The method allows selective labeling and ligation, without recourse to the use of protein-splicing elements. NCL=native chemical ligation.
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15 صفحه اولRefolding Process of Cysteine-Rich Proteins: Chitinase as a Model
Background: Recombinant proteins overexpressed in E. coli are usually deposited in inclusion bodies. Cysteines in the protein contribute to this process. Inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in E. coli. Hence, aggregated proteins should be solubilized and allowed to refold to obtain nat...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2013
ISSN: 1433-7851
DOI: 10.1002/anie.201304997